Topic: CNS demyelination

Microglia are key mediators in CNS demyelinating neurodegenerative diseases, controlling the disease environment by inhibiting or favoring CNS repair. Here we combine single-cell RNA-sequencing of cortical microglia and brain bulk RNA-sequencing, together with 3D brain imaging in experimental cuprizone demyelination to provide a longitudinal characterization of brain transcriptome and microglia signatures during disease progression and remyelination. First, brain RNA-sequencing of healthy, demyelination and remyelination states revealed upregulation of microglia genes involved in inflammation,

Painful diabetic neuropathy (pDN) is a serious, chronic complication affecting about 10-20% of diabetes mellitus (DM) patients, but the underlying cause remains unknown. Hints from risk factor assessment and literature point towards a role of oligodendrocyte and myelin homeostasis. Along this line, the main aim of the project is to identify adaptations occurring in the oligodendrocyte lineage due to the metabolic imbalances triggered by DM and determine which of these adaptations are crucial for the development of neuropathic pain. The high-fat high-sugar diet-induced type 2 DM mouse model is