Topic: confocal imaging

The concept of homeostasis is a central principle in many biological systems, and aims at maintaining systems in a functional state. In the brain homeostatic synaptic plasticity is a well-known mechanism to maintain neuronal networks in a functional homeostasis. Despite there has been a lot of research and characterization of this form of plasticity in animal models, it remains unknown, whether it is also expressed in the human brain. In this study, we investigated the impact of treatment with antiepileptic drugs on neurotransmission and functional properties of layer 2/3 pyramidal cells in th

TDP-43 is the hallmark protein of several neurodegenerative diseases, including Amyotrophic Lateral Sclerosis (ALS). This RNA-binding protein can form membrane-less condensates through liquid-liquid phase separation (LLPS). This biophysical process is considered to contribute to the regulation of TDP-43 functions but the precise mechanisms governing TDP-43 LLPS and its implications in homeostasis as well as pathology remain incompletely understood.In this study, we aimed to elucidate the influence of RNA-binding on TDP-43 dynamics and localization in vivo. By applying confocal imaging workflow

Astrocytes exhibit dynamic behavior in multiple sclerosis (MS), assuming both pro-inflammatory and anti-inflammatory phenotypes, yet their precise role in modelling the neuroinflammatory response in MS remains elusive. While mice are commonly employed for MS research, murine astrocytes fail to fully replicate the structural and functional complexity of their human counterparts, limiting the translation of findings from in vitro studies to the human context. Leveraging the unique characteristics of patient-derived iPSC astrocytes, this study aims to establish a platform for in vitro exploration