Topic: cortico-cortical projections

Astrocytes communicate bidirectionally with neurons by forming close contacts to synapses called tripartite synapses. Hundreds of thousands of synapses are embedded in a territory of single astrocytes, however, how astrocytes are functionally built into the neuronal circuits remains undetermined. To address this, we adopted the rabies virus (RV) for retrograde tracing of neuron-to-astrocyte connections in the mouse cerebral cortex. To sparsely and specifically transduce astrocytes with RV, a transposon vector expressing TVA, RV glycoprotein, and tdTomato under an astrocyte-specific promoter Gf

Neurodevelopmental disorders (NDDs) are a heterogeneous group of syndromes that typically manifest early in development. NDDs are characterized by intellectual disability/developmental delay (ID/DD) and resulting cognitive deficits. In our studies, we focus on Witteveen-Kolk Syndrome (WitKoS) characterized by mild ID, growth and feeding difficulties, autism spectrum disorder (ASD) and attention-deficit/hyperactivity disorder (ADHD) and some types of language developmental delay (Balasubramanian et al., 2021). This syndrome is caused by heterozygous loss-of-function mutations in SIN3A, a gene t