Topic: CRISPR-Cas9

Modern day “neurohackers” are radically self-experimenting, attempting genomic modification with CRISPR-Cas9 constructs and electrode insertion into their cortex amongst a host of other things. Institutions wanting to avoid the risks bought on by these procedures, generally avoid involvement with self-experimenting research. Modern day “neurohackers” are radically self-experimenting, attempting genomic modification with CRISPR-Cas9 constructs and electrode insertion into their cortex amongst a host of other things. Institutions wanting to avoid the risks bought on by these procedures, generall

Emmanuelle Charpentier and Jennifer Doudna explain the discovery and biochemical development of CRISPR-Cas9 as a programmable tool for modifying DNA. The scientific story begins with bacterial defence against invading genetic material and the role of tracrRNA in the CRISPR system. It proceeds through the reconstruction and simplification of the RNA-guided machinery and the demonstration that a chosen guide can direct DNA cleavage to a specified sequence. The lectures connect basic research on microbes and RNA with a method that transformed experimental genetics. They explain the molecular logi