Topic: dedifferentiation

Peripheral nerve degeneration (PND) regulated by Schwann cells in a hydrogen sulfide (H2S)-pathway-dependent manner is well known, but the underlying molecular properties of Schwann cells during PND are unclear. We investigated H2S signaling in in vitro- and ex vivo-dedifferentiated Schwann cells as unipotent stem cells by using global proteomics (LC-MS) and transcriptomics (whole-genome and small RNA-sequencing [RNA-seq]) methods. The multi-omics analyses identified several genes and proteins related to oxidative stress, such as Sod1, Gnao1, Stx4, Hmox2, Srxn1, and Edn1. The responses to oxid

While several studies have presented evidence that both mouse and human adult cortical astrocytes can dedifferentiate and form multipotent neurospheres after brain injury, other observations support that these neurospheres are formed by astroglia progenitors derived from subependymal zone (SEZ) resident neural stem cells which migrated to the cortex. In this study, we developed a new gliosis culture model composed by cells from the injured cortex, including reactive astrocytes, microglia, macrophages, and NG2 cells that mimic some characteristics of the injured brain but in a more controlled l