Topic: dLight3.8

The advent of dLight, a class of genetically encoded dopamine sensor, has significantlyadvanced our ability to monitor real-time dopamine dynamics in living animals. The success ofdLight1.3b with its superior dynamic ranges has prompted the development of subsequentiterations of this sensor to broaden the applicability of in vivo dopamine monitoring, particularlyin brain regions characterized by sparse dopaminergic signaling. In this study, we present asystematic and extensive in vivo characterization of novel dLight variants (dLight3.6 &dLight3.8) in comparison to the dLight1.3b parents s

Recent advances in biosensor technology have paved the way for an unprecedented level of inquiry into the spatiotemporal dynamics of neurochemical release in the brain. These sensors directly and specifically report synaptic signals as they are received by their receptors. dLight is a family of dopamine sensors with broadly tuned apparent affinity and dynamic range suitable to measure dopamine release between pM and µM range. They exhibit high affinity, millisecond response times, high signal-to-noise ratio (SNR), along with expression and targeting suitable for use in vitro and in vivo to tra