Topic: entorhinal cortex lesion

The entorhinal cortex lesion is a validated model for studying neuronal, dendritic and spine remodelling of dentate gyrus granule cells after denervation. Our research aims to investigate the effect of the layer-selective denervation of the dentate gyrus on the proliferation and differentiation of neurons in the granule cell layer, as the mechanisms that drive the remodelling due to the loss of input are not yet understood. Therefore, in anaesthetised adult male C57Bl/6 mice, the transection of the perforant pathway was performed, resulting in the layer-specific denervation of dentate granule

Denervation of brain regions is a common aspect of neurological diseases caused by traumatic brain injury or demyelination. Nevertheless, the mechanisms underlying lesion-induced reorganization of the affected brain regions is a matter of ongoing investigation.Using an entorhinal cortex lesion in mouse organotypic entorhino-hippocampal tissue cultures, we investigated denervation-induced adaptations in dentate granule cells, CA3 pyramidal neurons and their monosynaptic connection – the mossy fiber synapse. For functional and structural analysis of these cells, we employed single and paired who