Topic: Fiberphotometry

Activity of the insular cortex had been consistently suggested to be related with chronic pain. Of the sub-regions in the insular cortex, the posterior insular cortex (pIC) receives inputs from brain regions that receive primary sensory inputs. Anatomical changes of the pIC were reported in neuropathic pain conditions. However, how the activity of pIC neurons may be related with neuropathic pain remain to be investigated. We, therefore, investigated the role of pIC pyramidal neurons and neuropathic pain using optogenetics and fiberphotometry using male mice. We found that inhibiting the activi

The ability to switch between different behaviors is essential to all animals’ survival. The selection of a behavior is guided by multiple factors, such as sensory inputs, internal states, and memory, which suggests that regions across the brain are involved in the decision to switch between them. While whole-brain information is necessary to investigate the neural basis of behavioral switching, brain-wide imaging in behaving mice has proved challenging so far. We employed functional Ultrasound imaging (fUS) to record large-scale neural dynamics in head-fixed mice while simultaneously tracking