Topic: fluorescence calcium imaging

Neuronal ensembles, groups of simultaneously active neurons, are considered to be one of basic forms of information representation in the mammalian cortex. However, the mechanisms underlying the modulation of neuronal ensembles yet remain to be fully understood. Here, we investigated external input-dependent modulation of neuronal ensembles in cultured neuronal networks with modular connectivity mimicking the mammalian cortex. The modular neuronal networks were fabricated by growing cultured rat cortical neurons in microfluidic devices. A fraction of the neurons was stimulated using optogeneti

As part of the NeuChiP project aimed at developing Biological AI, we aimed to establish the plasticity properties of human-induced pluripotent stem cell (hiPSC) derived cortical networks. Objectives were to establish capacity and characteristics of human cultured networks.To achieve this, we engineered mouse cortical cultures and hiPSC-derived cortical neural networks into mature, specific configurations. Functional assessments were conducted with fluorescence calcium imaging and planar HD-MEA electrophysiological recording (3Brain - Duplex system).iPSC derived neural cultures were plated on c