Topic: fluorescence lifetime imaging

The Vps10p-Domain (Vps10p-D) receptor family consists of Sortilin, SorLA, SorCS1, SorCS2 and SorCS3. They mediate internalization and intracellular sorting of specific cargo in various cell types, including neurons, but underlying molecular determinants are incompletely understood. Deciphering the dynamic intracellular itineraries of Vps10p-D receptors is crucial for understanding their role in physiological and cytopathological processes. However, studying their spatial and temporal dynamics by live imaging has been challenging so far, as terminal tagging with fluorophores presumably impedes

Understanding the intricate network of molecular interactions responsible for synaptic plasticity remains a significant challenge in neuroscience research. Matrix metalloproteinase-9 (MMP-9) has been repeatedly implicated in the plasticity of excitatory synapses, notably in proBDNF processing. Our current study extends these findings by elucidating MMP-9's crucial role in dendritic spine plasticity through its involvement in the insulin-like growth factor I receptor (IGFIR) signaling pathway. Specifically, we demonstrate that MMP-9 is essential for IGFIR activation, a key process in long-term

Compelling evidence supports that exploiting both patient-derived and animal models is pivotal to reach a better understanding of Parkinson’s disease (PD) (DOI:10.1002/mds.28387). However, working with in vivo models can be challenging as most of them only partially reproduce the pathology at advanced age.We recently developed a novel zebrafish transgenic (tg) line expressing human full-length alpha-synuclein (aSyn) with an N-terminal mCherry tag named Tg(elavl3:mCherry-hsa.SNCA) which exhibit a series of PD-like features already in the larval stage. At 5 days-post-fertilization (dpf), Tg(elav