Topic: fluorescent calcium imaging

Transcranial focused ultrasound (tFUS) is a non-invasive neurostimulation modality capable of targeting deep brain regions with high spatial resolution. Despite its growing popularity, there is still a need to investigate the direct effect of its focused mechanical waves on the activation of the neuronal populations in specific brain areas in addition to the role that each of its physical parameters plays in this process. For this purpose, we have used fluorescent calcium imaging with fiber photometry to visualize the changes in the neuronal activity upon stimulation with tFUS. Fiber photometr

Fluorescent calcium imaging is an essential tool for measuring cellular activity, particularly as it applies to neurons and reporting action potentials. Genetically encoded calcium indicators are incredibly versatile and particularly useful for both in vivo and in vitro research. Depending on the application and use of indicators, the desired properties can drastically change from being very bright, to a very strong signal, to having fast dynamics, or any combination of the three. However, there are several disadvantages to using existing red shifted calcium indicators, those limitations being