Topic: Fura-2 calcium imaging

ePoster: 2 ePosters

The human-specific nicotinic receptor subunit CHRFAM7A (dupα7) reduces α7 nAChR function in human iPSC-derived and transgenic mouse neurons

Gokce Ilayda Gorgulu Soztekin, Vinita Jagannath, Stephanie Pons, Matthias Groszer, Uwe Maskos, Sigismund Huck, Petra Scholze · FENS Forum 2024

We investigated the impact of the human-specific protein CHRFAM7A (dupα7) on the function of α7 nicotinic acetylcholine receptors (nAChRs) in two different types of neurons: human induced pluripotent stem cell (hiPSC)-derived cortical neurons, and superior cervical ganglion (SCG) neurons, taken from transgenic mice expressing CHRFAM7A. The CHRFAM7A co-assembles with CHRNA7 but lacks a major part of the extracellular N-terminal ligand-binding domain. We assessed the function of homomeric and hybrid pentameric receptors with Fura-2 calcium imaging and three different α7-specific ligands: PNU2829

ePoster · Neuroscience

Temperature-sensitive transient receptor potential channels expressed in peripheral sensory neurons are functionally modulated by the tumor suppressor candidate 5

George Oprita, Dan Domocos, Vladimir Muzyka, Ana-Maria Taranciuc, Adelina Paduraru, Tudor Selescu, Alexandru Babes, Tudor Badea · FENS Forum 2024

Dorsal root ganglion (DRG) neurons are quintessential for peripheral pain transduction, a process mediated, among others, by certain members of the transient receptor potential (TRP) superfamily of ion channels. Among those, TRPA1, TRPV1, TRPM3 and TRPM8 have been linked to chronic pain and neuropathies. Tumor suppressor candidate 5 (Tusc5) plays a role in adipocyte function, but is also expressed in all major classes of DRG neurons. Tusc5 function in DRGs has not been investigated. We conducted Fura-2 calcium imaging and patch clamp experiments in cultured DRG neurons from knock-in Tusc5eGFP

ePoster · Neuroscience