Topic: GCaMP6f

This webinar will showcase new approaches for electrophysiological recordings using our silicon neural probes and surface arrays combined with diverse optical methods such as wide-field or 2-photon imaging, fiber photometry, and optogenetic perturbations in awake, behaving mice. Multi-modal recording of single units and local field potentials across cortex, hippocampus and thalamus alongside calcium activity via GCaMP6F in cortical neurons in triple-transgenic animals or in hippocampal astrocytes via viral transduction are brought to bear to reveal hitherto inaccessible and under-appreciated a

The superior colliculus (SC) is a major recipient of visual input in the mouse and controls innate approach and avoidance behaviours. Neurons in the superficial SC (sSC), which receives direct input from the retina, do not only process visual stimuli, but are also modulated by the animal’s running speed and pupil-linked arousal, similar to modulations observed in the primary visual cortex. While these observations were made during passive viewing of visual stimuli, a major purpose of vision is to guide behaviour. We therefore asked whether, in the context of a behavioural task, visual activity

Astrocyte calcium signals are region-specific and state-dependent across the sleep-wake cycle. However, their role in sleep regulation remains poorly understood. Since the pons is critical for sleep, especially rapid eye movement (REM) sleep regulation, we investigate the dynamics of pontine astrocyte calcium signals and their role in REM sleep induction in mice. We monitored pontine astrocyte calcium signals by expressing GCaMP6f under the control of gfaABC1D promoter and implanted optic fiber into the sublaterodorsal tegmental nucleus (SLD). Also, we simultaneously acquired cortical electroe