Topic: Gcamp8s

Individual’s survival relies on choosing optimal coping behaviors facing threats. The lateral habenula (LHb) is a pivotal structure when facing aversive stimuli as well as environmental cues predicting them. While it’s clear that individual LHb neurons together with LHb circuits are engaged in those process, how non-neural cells respond in similar scenarios remains an open question. Hence, we propose to study the involvement of LHb astrocytes during the encoding of aversive stimuli. Using in vivo expression of the genetically encoded calcium biosensor Gcamp8s and photometry-based recordings, w

Despite the axolotl’s exceptional ability to regenerate complex tissues, little research has focused on this phenomenon in the brain. We aim to understand whether a morphological repair observed during axolotl brain regeneration is accompanied by a functional restoration of neuronal circuits. To do so, we are establishing state-of-the-art neuroscience tools in our model system, namely GCaMP imaging of neuronal activity, behavioral assays and optogenetic stimulation. Using these methods, we will investigate the visuomotor circuit of the axolotl in intact, injured and regenerating conditions.Rec