Topic: gene expression programs

Chromatin remodelling plays an essential role in development, for instance by controlling gene expression during cell differentiation. Mutations of chromatin remodellers are often implicated in developmental diseases such as CHARGE syndrome, in which a heterozygous mutation of the chromatin remodeller CHD7 is the major driver of the disease. To investigate the role of Chd7 during brain development, we used a dorsal telencephalon-specific Chd7 loss-of-function mouse model and discovered Chd7 acts as a multi-lineage coordinator. Histological analysis of Chd7 mutant mice shows that upon ablation

Cell identity and states are dictated by gene expression programs, influenced by a range of epigenetic modalities. In the neuronal system, these mechanisms underlie various functions, such as maturation and memory formation, and their deficits are linked to various neurological disorders. Recent advancement of single-cell sequencing technologies, including scRNA-seq, has accelerated our understanding of the cellular diversity with respect to molecular profiles. However, existing high-throughput sequencing methods have an inherent limitation that they can primarily provide a static ‘snapshot’ o