Topic: GRABAdo

Molecular signals mediating systemic inflammation entering the brain parenchyma to induce sepsis-associated encephalopathy (SAE) remain elusive. In this study, we demonstrate that peripheral lipopolysaccharide (LPS)-induced systemic inflammation could rapidly increase the plasma adenosine concentration, peaking at 6 hours post LPS injection (hpi). Using in vivo 2P-LSM imaging of adenosine sensor GRABAdo, we observed elevated adenosine levels in the cortex of awake mice, also peaking at 6 hpi. These concomitant pathological events strongly suggest adenosine in the circulation can join forces to

ATP/Adenosine, a type of neurotransmitter, has recently attracted attention as damage-associated molecular patterns (DAMPs) and has been reported to play an important role in the induction of inflammation via inflammasome. On the other hand, there have been few reports on in vivo measurements of ATP and adenosine in the synaptic cleft and/or extracellular spaces.Generally, in vivo measurement of neurotransmitter content in the synaptic cleft and/or extracellular spaces has been performed mainly by invasive methods such as micro dialysis. However, recently, GPCR-activation-based (GRAB) sensor w