Topic: guide RNAs

Adenosine Deaminases Acting on RNA (ADAR) enzymes catalyse the conversion of adenosine (A) to inosine (I) within double-stranded RNA (dsRNA) molecules. This mechanism, known as A-I RNA editing, is able to modulate gene expression, RNA stability and protein function.Recently, new therapeutic approaches based on site-directed RNA editing (SDRE) leverage the recoding ability of ADAR to correct pathogenic G to A nucleotide mutations. SDRE allows for the manipulation of genetic information without causing damage to the genome. G-to-A point mutations make up nearly 30% of the pathogenic single nucle

Mammalian synapses facilitate high-frequency sustained neurotransmission through the fusion of synaptic vesicles (SVs) with the presynaptic membrane, followed by rapid recycling of SV machinery, including SNARE components, SV proteins, and membrane lipids over milliseconds to minutes. Visualizing these SV exo-endocytosis events is challenging due to their speed and nanoscale localization. To address this, we identified candidate SV proteins with abundant representation on SVs for precise labelling, aiming to enhance contrast. Conventional methods like over-expression, antibody-based labelling,