Topic: hCMEC/D3

Epidemiological studies put in evidence how the brain is emerging as an important target for adverse health effects of airborne pollutants (AP). We thus investigated the direct effect of AP on hCMEC/D3, astrocytes, microglia (MG) and neurons using the standard reference material of diesel exhaust particles (DEP). To study the DEP impact on purinergic signalling, we compared untreated primary MG with those incubated with DEP. Our data showed that DEP-activated MG generated much smaller ATP-induced Ca2+ waves, revealing a significant suppression of the receptor-evoked Ca2+ signals. The same resu

Increased levels of TNF-Related Weak Inducer of Apoptosis (TWEAK) are associated with neuroinflammation, especially during multiple sclerosis (MS), and the cytokine engenders permeabilization of in vitro blood-brain barrier (BBB) models. We sought to determine the effect of TWEAK on human brain microvascular endothelial cells (BMECs), specifically the hCMEC/D3 cell line. We used microarray analysis to investigate the transcriptomic changes of hCMEC/D3 induced by rhTWEAK incubation at three timepoints (3h, 12h, and 24h). We identified a set of differentially expressed genes (DEGs) probing the d