Topic: HEK293T cells

Adenosine Deaminases Acting on RNA (ADAR) enzymes catalyse the conversion of adenosine (A) to inosine (I) within double-stranded RNA (dsRNA) molecules. This mechanism, known as A-I RNA editing, is able to modulate gene expression, RNA stability and protein function.Recently, new therapeutic approaches based on site-directed RNA editing (SDRE) leverage the recoding ability of ADAR to correct pathogenic G to A nucleotide mutations. SDRE allows for the manipulation of genetic information without causing damage to the genome. G-to-A point mutations make up nearly 30% of the pathogenic single nucle

The application of biotin ligase or peroxidase fused with specific proteins or subcellular regions, combined with mass spectrometry-based quantitative proteomics, has become a potent approach for unravelling molecular interactions and determining the locations of native proteins based on their proximity. Recent applications of proximity-dependent biotinylation in neuroscience have yielded insights into the composition of sub-synaptic structures, including the synaptic cleft [1].Despite the effectiveness of proximity labelling methods in providing a global perspective on protein-protein interac

The molecular mechanism of the antimalarial artemisinin and its derivatives is not yet fully understood, although these compounds are successfully used against Plasmodium protists. Other biological effects (anti-cancer or antinociceptive effects) are also possible following administration of artemisinin. Considering that Transient Receptor Potential (TRP) ion channels are key signalling molecules in many cancer and pain pathways, the aim of this study was to test whether members of this family are among the molecular targets of artemisinin. The effect of artemisinin on wild type or mutant TRP