Topic: iGluSnFR3

Dendrites play a fundamental role in neuronal computation. It is thought that functionally defined regions of dendritic branches, termed ‘dendritic computational units’ are used to decode information transmitted by synaptic input. This information is encoded by the corresponding dendritic output, to perform complex neuronal computations. To investigate dendric input-output in vivo, it is necessary to record synaptic input and dendritic output simultaneously. To do this we perform simultaneous glutamate imaging and voltage imaging from the spiny dendrites of single Purkinje neurons in awake mic

A key question in neuroscience is how neurons compute their specialized output from their synaptic inputs. Another key question is whether this computation is shaped by non-linear dendritic integration. To answer these questions, it would be ideal to map synaptic activity while recording dendritic and somatic responses. We developed a strategy to simultaneously measure glutamate synaptic release and postsynaptic responses in L2/3 pyramidal neurons in the mouse primary visual cortex. We sparsely coexpressed two sensors: the green membrane sensor iGluSnFR3, reporting presynaptic release of gluta

Amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD) are adult-onset neurodegenerative disorders that share common pathology and genetic causes. In many cases the RNA binding protein TDP-43 aggregates in the cytoplasm leading to the loss of its nuclear functions, including as a splicing repressor. This results in the erroneous inclusion of intronic sequences known as cryptic exons (CEs) in several mature mRNAs. Recently, it has been shown that TDP-43 depletion leads to CE inclusion in UNC13A, which encodes a protein crucial for priming synaptic vesicles for release. The UNC13A