Immunofluorescence

Light-Sheet Fluorescence Microscopy (LSFM) is an excellent tool to study neuronal populations in large samples such as the whole mouse brain, thanks to its inherent three-dimensional nature and its high resolution down to the single cell. In this work we report on our latest technological developments in both hardware and software and their applications in several projects aiming at reconstructing the neuronal organization in both the human cortex and in whole mouse brain.Our latest LSFM setup is capable of simultaneous excitation and acquisition at four wavelengths, thus enabling fast reconst

…electron microscopy, PAS staining and immunofluorescence with natural IgMs and anti-glycogen synthase (GS) antibodies. However, the methods employed involved the use of a large sample volume for each staining, which hindered wasteosomes characterization. In the present work, we examined a new method to

Progress in brain research is widely based on experimental approaches having the potential to advance the knowledge on fundamental biology. In particular, the increasing availability of data obtained by experimental techniques has boosted the development of data-driven mathematical models reproducing neuronal circuits. While cellular and subcellular resolution data have been extensively collected in animal models, experimental constraints have limited the acquisition of information about human neuronal cytoarchitectures and morphologies. In this context, high resolution data can be efficiently

Choline Acetyltransferase (ChAT) serves as a reliable marker for motoneurons, including those within the oculomotor (OCM) system located in the brainstem. Although ChAT activity increases in spinal cord motoneurons during postnatal development, the evolving expression profile of this enzyme within the OCM system across various life stages remains unexplored. Previous investigations have indicated an elevation in nerve growth factor (NGF) expression in the spinal cord during postnatal development, mirroring ChAT patterns. Ocular motoneurons exhibit a distinct dependence on NGF due to the consti

Brain ageing in mice leads to the progressive appearance of degenerative granular structures frequently referred to as “PAS granules” because of their positive staining with periodic acid-Schiff (PAS). It has been reported that these granules contain neo-epitopes that can be recognised by natural IgM auto-antibodies, and proposed that they intervene in a brain cleaning mechanism. Some preliminary studies seem to indicate that these neo-epitopes are also present in other structures than PAS granules, so the aim of this work has been to study and characterize the presence of neo-epitopes in mice