Topic: induced neurons

Genetic reprogramming techniques have been used with increasing success to generate human neurons from adult somatic cells. Induced pluripotent stem cell (iPSC) derived neurons have been shown as an effective way to investigate normal and pathological neuronal development. Additionally, transcription factor-based direct conversion of fibroblasts into induced neurons (iNs) has been established. Such iNs have been shown to retain age-related epigenetic information of the donor and therefore are viewed as a more appropriate model system for studying human neuronal aging and related pathologies. I

The aim of this work is to analyze the roles of the adaptor protein p140cap in human nervous system, at neuronal and synaptic levels, using induced neurons from human induced pluripotent stem cells (iPSCs). Studies on mice models have demonstrated how p140cap is involved in the regulation of synaptic functions, especially in the maturation, stabilization, and remodeling of dendritic spines. This protein is also implicated in plasticity phenomena, such as LTP, LTD, learning, and memory, due to its direct interaction with the GLUN2A subunit of NMDAR. The p140cap protein is encoded by the SRCIN1

Primary cilia are sensory organelles understood to play important roles throughout development, as evidenced by the spectrum of neurodevelopmental phenotypes observed in instances of ciliary dysfunction (termed ciliopathies). Recent research indicates that in the adult brain, primary cilia may play roles in excitability, maintaining synaptic connectivity, and even form specialised synapses capable of altering the transcriptional landscape of neurons.To further understand the role of cilia in postmitotic neurons, we generated human induced pluripotent stem cell lines (hiPSCs) with NPHP1-/- and

Age-related neurodegenerative diseases are becoming more prevalent as human lifespan increases. Modeling human aging is challenging in the lab. We use transdifferentiation via an all-in-one lentiviral vector to directly reprogram human donor-derived fibroblasts into induced neurons (iN). The advantage of this methodology is the maintaining of age and disease-related signatures of the donor cells as iNs bypass the intermediary cell rejuvenation.Huntington’s disease (HD) is an autosomal dominant age-related neurodegenerative disorder caused by a mutated Huntingtin (HTT) gene containing an expans