Topic: juxtacellular recording

To explore how supragranular cortical pyramidal cells (Pyrs) are recruited during natural sleep, we applied a method of juxtacellular recording/labeling in freely moving rats. We asked how different Pyrs activate during sleep events such as down-to-up-state transition (DUt), spindle (SO), and REM sleep-related theta oscillations (ThO). Based on the polarity of the down-states (DS) recorded along with the cell firing, we divided Pyrs as superficial (negative DS, layer 2 – L2, n=35) or deep (positive DS, layer 3 – L3, n=35). Most of the Pyrs showed very sporadic firing behavior, with a lower ave

The dorsal tegmentum including the ventrolateral PAG (vlPAG) and dorsal raphe nucleus (DRN) plays a critical role in controlling anxiety, fear memory formation, autonomic processes and most particularly, it is involved in descending modulation of pain processing. It has been shown that different neuron types, such as dopaminergic and serotonergic cells are part of this circuitry – besides the glutamatergic and GABAergic neurons – however their functions remained unclear. Here, we explored the neuronal activity in the vlPAG and DRN in urethane-anesthetized mice in response to different types of

The Locus coeruleus (LC), the main noradrenaline-producing nucleus in the brain, is affected in a wide range of neurological conditions such as Parkinson's disease and Alzheimer's disease. Notably, the locus coeruleus-noradrenergic (LC-NA) system can be a therapeutic target through Vagus Nerve Stimulation. Transcutaneous auricular Vagus Nerve Stimulation (taVNS), an alternative technique that stimulates the auricular branch of the vagus nerve, is being studied as it’s less invasive, yet the nuanced neurocircuitry linking this branch of the vagus and LC remains unelucidated, as does the optimal