Topic: KIF5A

Multiple neuronal functions require decentralized processes including activity-dependent localized protein synthesis. However, how local protein synthesis drives the dendritic remodeling is still elusive. Kinesin superfamily proteins (KIFs) are microtubule-based motor proteins that transport various types of cargos to a specific area where they function. This intracellular transport system is essential for neuronal morphogenesis, function, and survival. KIF17 and KIF5A are abundantly expressed in neurons and have been suggested to transport NMDAR and AMPAR in neuronal dendrites, respectively.

Spinal muscular atrophy (SMA) is a neurodegenerative disease characterized by spinal cord and brainstem motor neuron (MN) loss. Axonal transport defects are a common hallmark of MN degeneration and KIF5A, a major component of the axonal transport machinery, has been associated with MN diseases, but its role in SMA has not been elucidated yet. Our aim was to investigate a possible correlation between KIF5A and SMA pathogenesis. Therefore, Western Blot, qRT-PCR and immunohistochemistry analysis were performed to investigate KIF5A and its adaptor protein complex Miro1/Milton expression in the spi