Topic: lncRNAs

Study objective was to evaluate whether seizure-inducing chemoconvulsants could induce dysregulation of non-coding RNAs (ncRNAs), similar to epileptogenic brain insults and seizures observed in-vivo.Rat primary cortical cultures were exposed to 11 chemoconvulsants (4-aminopyridine, amoxapine, bicuculline, chlorpromazine, donepezil, kainic acid, pentylenetetrazol, picrotoxin, pilocarpine HCl, SNC80, strychnine HCl) for 24 h, followed by extraction of RNA for sequencing. Salmon tool quantified ncRNAs. DESeq2 analyzed differential expressions. qPCR validations for samples exposed to amoxapine, ch

Glucocorticoid signalling serves as a central regulator of the body's stress response and has been associated with several diseases affecting the central nervous system (CNS), such as major depressive disorder or Alzheimer’s disease. While the cell type-specific effect of glucocorticoids in the CNS has been studied previously, our understanding of how glucocorticoids act on astrocytes and their impact on neuronal network plasticity is limited. It's important to note that only 1.5% of the genome encodes proteins, while the remaining 98.5% consists of non-coding RNAs (ncRNAs) implicated in vario