Topic: long non-coding RNAs

The vast majority of the human genome consists of noncoding elements, including introns, intergenic regions, and various types of noncoding RNAs (ncRNAs). As such, protein-coding genes represent only 1.5% of the transcriptome while the remaining 98,5% are ncRNAs. Long non-coding RNAs (lncRNAs) form the largest class of ncRNAs and are characterized by a length of more than 200 nucleotides. The role of lncRNAs in the central nervous system (CNS) is still not well understood. In this project we identified a novel lncRNA that we named CNS-LNC due to its specific expression pattern in the adult bra

Numerous long non-coding RNAs (lncRNAs) play pivotal roles in nervous system development and the modulation of neuronal activity, making them crucial physiological players and potential vulnerability points for neuropsychiatric disorders. MALAT1 stands out as one such lncRNA, exerting control over splicing events by sequestering specific SR-family splicing factors in nuclear inactive compartments such as the nuclear speckles, thereby impeding their action on target transcripts. Notably, nSR100, a member of the SR-family, serves as a key splicing regulator, facilitating the generation of neuroL

Non-coding RNAs are transcripts that do not encode proteins and comprise 98.5% of the human transcriptome. Among these, long non-coding RNAs (lncRNAs) have been implicated in various biological functions, including the control of gene expression and translation. The central nervous system (CNS) harbors a diverse array of lncRNA transcripts, yet the functions of many remain largely unexplored. In this study, we employed bulk and single-cell RNA sequencing approaches using human and mouse brain tissue to identify novel lncRNAs in both neuronal and non-neuronal cells, potentially associated with

Aim: Over the past decade, our understanding of the transcriptional landscape in the brain has undergone a dogma-challenging expansion, revealing the intricate role of non-protein-coding RNAs (ncRNAs) in neural function, development, and disease. A large number of ncRNAs have been discovered and implicated in molecular regulatory processes involved in neural homeostasis and activity and seem to play crucial roles in neurodegenerative disorders and cancer. This study aims to assess the efficacy of in situ hybridization (ISH) in profiling long non-coding RNAs (lncRNAs) and circular RNAs (circRNA

Aims: Large-scale changes in epigenetic-mediated regulation of gene expression is known to drive the development of temporal lobe epilepsy (TLE) after a precipitating brain insult. Although long non-coding RNAs (lncRNAs) are key modulators of RNA processing, their functional involvement in epilepsy pathogenesis remains largely unexplored. Here, we perform the first comprehensive profiling study of lncRNAs during epileptogenesis and assess their utility as novel drug targets. Methods: To study lncRNA dysregulation, Next Generation Sequencing was performed on hippocampal tissue collected during

Alterations of N6-methyladenosine (m6A) RNA modifications are responsible for a platter of structural and functional changes in healthy and cancerous cell states. Even though several studies have linked m6A to the onset and development of tumors, very few have examined transcriptome-wide m6A landscape of long non-coding RNAs (lncRNAs) in cancerous cells, including gliomas. This work aims to explore and elucidate lncRNA m6A alterations and expression in glioma stem cells, U87-MG cells and glioma patients. We utilized four cell lines (NCH421k, NCH644, NCH690, U-87 MG), along with 16 glioblastoma