Topic: LUHMES cells

Amyloid beta1-42(Aβ1-42) peptide, a major pathological component of Alzheimer's disease(AD), is known to locate and accumulate in the mitochondria.Research has demonstrated that nuclear transcription factors(nTF) play a direct or indirect role in the transcriptional regulation of mitochondria, which lose function in the early stages of neurodegenerative diseases.Based on this information, our study investigates the interactions between Aβ and nTFs in mitochondria.In this study, the interaction of 48 nTFs with Aβ was examined using a nTF-interaction kit. Mitochondrial lysate isolations were per

The increasing environmental spreading of perfluoroalkyl substances (PFASs) raises concerns for theirimpact on human health. PFASs are synthetic chemicals widely used in industrial and consumerproducts to make surfaces repellent to water and lipids. Once ingested or inhaled, PFASs arebioaccumulated in the blood, liver, kidney, and brain of various species with an elimination half-lifein humans of 3-5 years. In a previous publication, we highlighted for the first time that theaccumulation of PFASs can alter the development of human dopaminergic neurons (Di Nisio et al.,2022). This finding, toge

Sturge-Weber syndrome (SWS), a rare neurovascular disorder associated with a GNAQ gene mutation, presents distinct clinical features, including a facial port-wine birthmark, glaucoma, and various neurological symptoms. Despite these characteristics, the molecular complexity of neurovascular cross-talk remains poorly understood. This study aims to unravel Gαq regulation in neural cells, focusing on surface marker expression. To study surface marker regulation, CRISPR-mediated Gαq knockouts were generated in Lund human mesencephalic cells (LUHMES; neural) and pluripotent stem cells (embryonic).