Topic: mApoE-PA-LIP

Epidemiological studies put in evidence how the brain is emerging as an important target for adverse health effects of airborne pollutants (AP). We thus investigated the direct effect of AP on hCMEC/D3, astrocytes, microglia (MG) and neurons using the standard reference material of diesel exhaust particles (DEP). To study the DEP impact on purinergic signalling, we compared untreated primary MG with those incubated with DEP. Our data showed that DEP-activated MG generated much smaller ATP-induced Ca2+ waves, revealing a significant suppression of the receptor-evoked Ca2+ signals. The same resu

Recently, several disorders of the central nervous system (CNS), including Alzheimer's disease (AD) and neuroinflammation have been associated with dysfunction of the neurovascular unit (NVU). As a result, there is an increased demand for therapeutic tools able to act at the NVU. We studied liposomes functionalized with apolipoprotein-E and phosphatidic acid (mApoE-PA-LIP), previously used to promote Aβ removal across the blood-brain barrier (BBB) in an in vivo model of AD, with memory improvement. We performed calcium imaging to study ATP-evoked intracellular Ca2+ waves in hCMEC/D3 and in ast