Topic: mCherry

Higher order sensory information is typically relayed through the posterior thalamustowards the cortex. Using a transgenic mouse strain, we identified a neuronpopulation in the posterior thalamic nuclear group, based on their transient embryonicexpression of secretagogin, a calcium sensor protein. Anterograde tracing wasperformed by using adeno-associated viral vectors carrying the mCherry transgene in12-16 weeks old Scgn-Cre mice and imaging was performed after standardimmunohistochemistry on a ZEISS 780 CLSM platform. Other animals received AAVparticles carrying Cre-dependent DREADD expressi

Compelling evidence supports that exploiting both patient-derived and animal models is pivotal to reach a better understanding of Parkinson’s disease (PD) (DOI:10.1002/mds.28387). However, working with in vivo models can be challenging as most of them only partially reproduce the pathology at advanced age.We recently developed a novel zebrafish transgenic (tg) line expressing human full-length alpha-synuclein (aSyn) with an N-terminal mCherry tag named Tg(elavl3:mCherry-hsa.SNCA) which exhibit a series of PD-like features already in the larval stage. At 5 days-post-fertilization (dpf), Tg(elav