Metabolomic analysis

Embryonic neurodevelopment, which occurs in the sterile uterine environment, has recently been shown to be modulated by signals from the maternal gut microbiota. We hypothesised this may occur through microbial signalling to radial glial (RG) cells, which generate cortical neurons, and function as a scaffold along which new-born neurons migrate. Therefore we aimed to study the effect of maternal gut microbiota depletion during pregnancy on RG-led neurodevelopment. Female mice were treated with an antibiotic cocktail prior to and throughout pregnancy to deplete their gut microbiota. Embryonic b

…Metabolomic analysis offers a promising, non-invasive approach to identify potential biomarkers for improved diagnostic precision.Cerebrospinal fluid (CSF) and serum samples from 24 healthy controls, 48 MS patients, and 20 Non-MS patients with a range of diverse conditions, including infections, autoimmune disorders, inflammatory issues, and other neurological disorders were collected during lumbar puncture. NMR analysis on a

We recently identified Protein kinase N1 (PKN1) as a negative regulator of neuronal AKT activity during postnatal cerebral development. Given the pivotal role of the AKT pro-survival signalling cascade in ischemia/reperfusion, our investigation aimed to determine whether PKN1-mediated AKT inhibition remains relevant during pathological conditions in the mature brain. Employing adult wild type and global Pkn1 knockout (Pkn1-/-) mice, we observed that Pkn1 knockout indeed imparts a protective phenotype in both, in vitro and in vivo stroke models. However, this was not associated with enhanced AK