Topic: Methylcobalamin

Aim: Methylcobalamin (MeCbl) is known to promote peripheral nerve regeneration and polarize macrophage to M2. In recent years, it has been reported that the regeneration of peripheral nerves and the polarization of macrophages to M2 are important for bone formation. The purpose of this research is to examine the effects of MeCbl on bone formation via peripheral nerves and macrophages. Methods: Effects of MeCbl were evaluated using rat skull defect models. Briefly, we created a bone hole in the rat skull, and evaluated the bone formation rate in the defect area in a group that received continuo

Aims: To investigate the effects of methylcobalamin (MeCbl) on angiogenesis after peripheral nerve injury.Methods: For in vivo experiments, we used eighteen 6-week-old male Wistar rats. The left sciatic nerve was excised and bridged using the decellularized nerve. Then, rats were randomly divided into MeCbl treated and saline treated groups. At postoperative 7 days, nerves were harvested and evaluated for immunohistochemistry. For in vitro experiments, scratch assay, and tube formation assay were evaluated in the presence or absence of MeCbl using human umbilical vein endothelial cells. Additi

In this study, we aimed to explore methylcobalamin (MeCbl), a common treatment for peripheral nerve injuries, and its impact on macrophage phenotypes (inflammatory or anti-inflammatory) at nerve injury sites and the underlying mechanism. Using a sciatic nerve crush injury model in 6-week-old Wistar rats, we administered either saline (control group) or MeCbl (MeCbl group) via osmotic pumps for sustained systemic delivery. We assessed macrophage phenotypes in transverse sections of the sciatic nerve up to 14 days post-surgery using fluorescence immunostaining. In vitro, we induced inflammatory