Topic: molecular layer interneurons

Locomotion in complex environments depends on the precise timing and active control of single paw movements in order to adapt steps to surface structure and coordinate between paws. Control of motor timing crucially depends on the cerebellum, which has been shown to provide signals necessary for movement initiation and termination. While recent work found single-paw kinematics during locomotion to be encoded in cerebellar Purkinje cells, precise, event-related activity in the cerebellum in more complex environments is less well understood. Moreover, whether precise action timing in complex env

Complex motor behaviors involve the precise coordination of different body parts. While motor coordination has been extensively studied in expert animals, the behavioral and neural processes involved in its emergence through learning are still not well understood. To answer this question, we combined longitudinal behavioral analyses with population recordings from the cerebellum, which is a key region for movement coordination and motor learning. We trained mice to walk on a motorized runged treadmill over multiple days while Ca2+-activity was recorded from cerebellar molecular layer interneur

Understanding dendritic integration in relation to its excitatory and inhibitory inputs is critical to deciphering neuronal data processing. Upon sensorimotor stimulus, the cerebellar Purkinje neuron (PN) receives excitatory inputs from a single climbing fiber (CF), thousands of parallel fibers (PFs), and feed-forward inhibitory inputs (FFI) from molecular layer interneurons (MLIs). This study aims to understand the dendritic interplay of MLI inhibitory inputs, shaping excitatory inputs upon air puff stimulation in awake mice.We labeled MLI axons with GCaMP6 and PN with the voltage-sensitive d

The cerebellum is an area of the brain involved in sensory-motor adaptation, movement coordination and higher cognitive processes. Within the cerebellar cortex, mossy fibres (MFs) convey sensorimotor input to a dense layer of granule cells (GrCs). GrCs relay this information through parallel fibre (PF) projections that synapse with Purkinje cells (PCs), the sole output of the cerebellar cortex, and molecular layer interneurons (MLI), which provide feedforward inhibition of PCs. Accordingly, PCs compute incoming sensorimotor patterns and send inhibitory outputs that rapidly adjust motor movemen