Topic: Mosaic Analysis with Double Markers

The superior colliculus (SC) is a dorsal midbrain structure known for its roles in multisensory integration and motor responses and its dysfunction has been linked to neurodevelopmental diseases. SC is a highly organized structure composed of distinct laminae of diverse neuronal and glial cell types. However, the precise developmental principles instructing the generation of SC cell-type diversity are still unknown. To investigate this, we pursued lineage tracing using genetic MADM (Mosaic Analysis with Double Markers)-based clonal analysis with single cell sequencing (MADM-CloneSeq) and in si

The mammalian neocortex is populated by different neuronal and glia cell types. During development, excitatory projection neurons and certain glia lineages emerge from radial glial progenitors (RGP). Impairments in RGP progenitor behavior and lineage progression can affect neuron production and maturation, leading to cortical malformations such as micro- or macrocephaly. The Notch signaling pathway instructs stem cell self-renewal and differentiation in a variety of tissues, including the brain. The Notch1 receptor is expressed in RGPs during embryonic development and maintains the progenitor

The cerebral cortex is composed of an extraordinary number of neuronal and glial cell types assembling into cortical circuits that account for cognitive abilities. Remarkable heterogeneity in the spectrum of cortical cell types has been described on the basis of global transcriptome analysis. How cell-type diversity is generated by radial glia progenitor (RGP) cells during development is however still poorly understood. Here we focus on the Rptor/mTORC1 signaling pathway and its role in RGP cell lineage progression and cortical neurogenesis. By using Mosaic Analysis with Double Markers (MADM)

In the developing cerebral cortex, radial glial progenitors (RGPs) produce all excitatory neurons and glia. Using Mosaic Analysis with Double Markers (MADM), we established a quantitative framework of cortical RGP lineage progression at single-cell resolution in vivo. The extent to which these quantitative aspects of RGP lineage progression are conserved in vitro is unclear. Here we established MADM in embryonic stem cells in order to contrast RGP lineage progression in vivo and in cortical organoids. We found that organoid RGP division behavior was less temporally stereotyped, with both persi