Topic: MPP+

Microglial reactivity is linked with the loss of dopaminergic (DA) neurons in Parkinson’s disease (PD). In this context, we propose that calcium, potentially released by DA dying neurons to the extracellular space, may contribute provoking changes in microglial polarization.Postmortem samples from PD patients exhibited increased microglia-neuron interactions in the Substantia Nigra (SN) when compared with control individuals, indicating a polarization of microglia in this pathological scenario. Besides, MPTP-treated parkinsonian mice also revealed a significant number of polarized microglia to

1-Methyl-4-phenyl-pyridinium (MPP+) and 6-hydroxydopamine (6-OHDA) are the predominant neurotoxic agents used in the research of Parkinson's disease (PD). The mTORC2 is a significant signaling pathway whose specific functions and mechanisms remain partially elusive, especially in the context of PD-related oxidative stress. We aimed to determine the interconnection between mTORC2 and oxidative stress in MPP+ and 6-OHDA neurotoxic models of PD. All experiments were performed on the human neuroblastoma cell line, SH-SY5Y. Cells were divided into two groups: control (consists of control cells and