Topic: multiphoton imaging

Neuromodulators adjust sensory processing and synapses are key control sites for such plasticity. Less clear is how neuromodulation alters the amount of information that synapses transmit through a circuit. To understand the operation of the “vesicle code” the experimenter needs to isolate signals from individual active zones with a resolution of single vesicles while observing or controlling the incoming signal. This has not been possible using electrophysiology but can now be achieved by multiphoton imaging of the glutamate reporter iGluSnFR in the retina of larval zebrafish. This approach

The interplay between spontaneous and sensory-driven activity plays a critical role in regulating neuronal structure and function of neuronal circuits during development. Here, we investigated how deprivation of visual inputs (dark rearing) during the early stage of development affects sensori-motor integration at the behavior and neuronal circuit level. Due to the intrinsic characteristic of the mammalian brain, it has not been possible to analyze simultaneously the impact of neuronal activity in shaping circuits across neuronal populations in distinct brain areas. To overcome this limitation

Serotonin is a critical regulator of cortical neurodevelopment whose perturbation is associated with neurodevelopmental disorders. Here, we explore its neurodevelopmental dynamics and how they dictate the emergence of cortical population dynamics by combining longitudinal multiphoton imaging of serotonin and calcium sensors with slice electrophysiology in the somatosensory cortex of interneuron-labelled mice with a genetic (knock-out), or pharmacological (postnatal fluoxetine) developmental disruption of serotonin dynamics. We have characterised global and local cortical serotonergic fluctuati