Topic: multiplexed in situ hybridization

The Xenopus laevis frog shows a wide range of motor behaviors during metamorphosis, transitioning from tail-based escape swimming, to free swimming, and finally, limb-based movement. This swim-to-limb transition allows us to study in a single organism how motor and interneurons in the spinal cord generate these behaviors. Here, utilizing advances in genetic manipulation and behavioral tracking, we dissect the molecular features and contribution of each spinal cord cardinal class to swim versus limb behavior over frog metamorphosis.We devise a high-throughput CRISPR screen, in which we knock ou

Alzheimer’s disease (AD) is a neurodegenerative disorder with complex pathological manifestations and is the leading cause of cognitive decline and dementia in elderly individuals. A major goal in AD research is to identify new therapeutic pathways by studying the molecular and cellular changes in the disease, either downstream or upstream of the pathological hallmarks. In this study, we present a comprehensive investigation of cellular heterogeneity from temporal cortex region of 40 individuals, comprising healthy donors and individuals with differing tau and amyloid burden. Using single-nucl