Topic: network synchronization

Haploinsufficiency of the CACNA1A gene, encoding the pore-forming α1 subunit of P/Q-type voltage-gated calcium channels, is associated with a clinically variable phenotype ranging from cerebellar ataxia, to neurodevelopmental syndromes with epilepsy and intellectual disability. To understand the pathological mechanisms of CACNA1A loss-of-function variants, we characterized a human neuronal model for CACNA1A haploinsufficiency, by differentiating isogenic induced pluripotent stem cell lines into glutamatergic neurons, and investigated the effect of CACNA1A haploinsufficiency on mature neuronal

Aims Microelectrode arrays (MEA) are commonly used in neuroscience as a device for monitoring neuronal firing and synaptic activity in vitro from 2D and 3D neuronal cultures and brain slices. MEA recordings serve as powerful functional assay for neuroscience, but this is only applicable if it does not outstrip our ability to analyze the data. Here we present the MEASpikeR package, an R toolbox specifically designed for analyzing and exploring in vitro neuronal spike activity and field post synaptic potential (fPSP) recorded with the MED64 system (AlphaMED Scientific). Methods MEASpikeR consist

Long-term recordings in patients and animal models reveal circadian and multidien (multiple days) rhythmicity in epilepsy. The underlying mechanisms of these cycles are not known. We are interested in predicting the onset of epileptic seizures over the scale of days. To this end, we performed chronic telemetric and single-unit recordings in epileptic rats (pilocarpine model). The primary focus of our investigation lies in the study of large network discharges known as interictal spikes (IS), closely associated with epileptic seizures.As previously shown, in our recordings the number of seizure