Network topology

…To reveal microscale network activity, we investigated age-related differences in the functional connectivity and network topology. We cultured human neurogenin 2 (NGN2) iPSC-derived neurons with human astrocytes on 64-electrode MEAs (n=15 cultures). We performed 10-minute recordings weekly from days-in-vitro (DIV) 14-35 and analysis with MEA-NAP (https://github.com/SAND-Lab/MEA-NAP/). We applied one-way ANOVA and

…study the mesoscale dynamics and the network topology of neuronal ensembles across different levels of consciousness, focusing on wakefulness, anesthesia, and awakening. The data were acquired by two chronically implanted multielectrode arrays (Utah 96), placed in the dorsal prefrontal cortex (dPFC) and dorsal premotor cortex (PMd) of two

In sexually reproducing species, males and females respond to environmental sensory cues and transform the input into sexually dimorphic traits. Yet, how sexually dimorphic behavior is encoded in the nervous system is poorly understood. We characterize the sexually dimorphic nociceptive behavior in C. elegans – hermaphrodites present a lower pain threshold than males in response to aversive stimuli, and study the underlying neuronal circuits, which are composed of the same neurons that are wired differently. By imaging receptor expression, calcium responses and glutamate secretion, we show tha