Topic: neurite elongation

Vacuolar-type adenosine triphosphatase (v-ATPase) is a multimeric complex that drives the transport of hydrogen ions upon ATP hydrolysis. At lysosomal compartments, v-ATPase mediates the luminal acidification necessary for catabolic processes, such as endocytic degradation and autophagy. Autophagy is particularly relevant for neurons, due to their post-mitotic nature, polarized morphology and high protein load sustaining synaptic activity and plasticity. v-ATPase is additionally expressed by synaptic vesicles, where it creates the proton gradient driving neurotransmitter loading. Alterations i

Some studies demonstrate the beneficial role of high doses of vitamin B12 (VitB12) supplementation for the recovery from specific neurological impairments; however, the detailed mechanisms are not clearly elucidated. With this in mind, we employed an in vitro cell model made of hydrogen peroxide-insulted differentiated-SH-SY5Y (exhibiting a neuron-like phenotype) to study the effect of both a high and the physiological dose of VitB12 during recovery. The physiological dose of VitB12 showed an improvement in the cell count (although cell viability, evaluated by MTT, was comparably decreased in