Topic: Neuroprogenitor cells

ePoster: 2 ePosters

Characterizing human-derived neuronal network using high-density MEAs and proteomics: In-vitro model for neurodevelopmental disease

Lorenzo Muzzi, Ilaria Musante, Simona Baldassari, Martina Bortolucci, Niccolò Callegari, Andrea Petretto, Federico Zara, Paolo Scudieri · FENS Forum 2024

While dissociated cultures of rodent neurons on microelectrode arrays (MEAs) have become a prominent method for studying neuronal network properties, establishing human neuronal cultures with mature electrophysiological features compatible with MEAs has posed a significant challenge. The aim of this study was to establish a reliable protocol for generating human-derived neuronal networks on High Density Microelectrode Arrays (HD-MEA) and to characterize their electrophysiological features for potential applications in disease modeling and therapeutic testing. Human-induced pluripotent stem cel

ePoster · Neuroscience

Neuropilin-mediated Sema 3 signaling is crucial for chick spinal cord neuroprogenitor cells to exit the cell cycle and protect early-born neurons from apoptosis

Rita Varga, Angelika Varga, Kirsten Roberts, Peter Szucs, Zoltan Meszar · FENS Forum 2024

After neurulation, neuroprogenitor cells differentiate into postmitotic neurons that migrate away from the ventricular zone and form a laminated structure in the spinal cord. Semaphorins are crucial for neuronal differentiation, particularly in axon pathfinding. Although semaphorin receptors are expressed in the ventricular zone of the developing spinal cord, their role in the neuronal cell cycle is not yet clear. Our study suggests that Sema 3 and their neuropilin receptors may be involved in a process that we disrupted using in-ovo electroporation. We overexpressed dominant-negative forms of

ePoster · Neuroscience