Neuronal correlates of social and spatial cues in the mouse retrosplenial cortex
In this study, we investigate how contextual memory is shaped by observational fear learning during encoding and recall. We are imaging neurons in layer 2/3 of the retrosplenial cortex in GCaMP6s mice with two-photon Ca2+ imaging using a head-fixed observational fear learning paradigm in virtual reality. The animals are surrounded by three screens and run on a running wheel as they travel through 3 different VR environments. In two of the environments, we play recordings of mouse vocalisations emitted while they were receiving foot shocks, and a neutral sound in the third environment. A single