Mapping of neuronal populations with light-sheet fluorescence microscopy
Giacomo Mazzamuto, Irene Costantini, Danila Di Meo, Franco Cheli, Michele Sorelli, Samuel Bradley, Josephine Ramazzotti, Curzio Checcucci, Alessandra Franceschini, Ludovico Silvestri, Francesco Saverio Pavone · FENS Forum 2024
Light-Sheet Fluorescence Microscopy (LSFM) is an excellent tool to study neuronal populations in large samples such as the whole mouse brain, thanks to its inherent three-dimensional nature and its high resolution down to the single cell. In this work we report on our latest technological developments in both hardware and software and their applications in several projects aiming at reconstructing the neuronal organization in both the human cortex and in whole mouse brain.Our latest LSFM setup is capable of simultaneous excitation and acquisition at four wavelengths, thus enabling fast reconst
ePoster · Neuroscience