Topic: optogenetic inhibition

Understanding how cortical circuits generate complex behavior requires investigating the cell types that comprise them. Here, much effort has been focused on inhibitory neuron types but the functional roles of distinct classes of excitatory pyramidal neurons (PyNs) are less well un-derstood. We, therefore, used widefield imaging to measure the cortex-wide activity of distinct PyN types and investigated their functional role in mice that performed an auditory decision-making task. We used two mouse lines, expressing the calcium indicator GCaMP6s in two major PyN types: FezF2 for pyramidal-tract

Optogenetic inhibition of excitatory subpopulations of the hippocampus has been suggested as a new approach in the treatment of temporal lobe epilepsy (TLE), one of the most common types of drug-resistant epilepsy. While this approach is promising, its application in animal models has proven to be a challenge. This can be attributed to the numerous parameters involved in both the optogenetic stimulation setup and the neuronal environment. These are difficult to investigate and optimize in limited in vivo experiments. Therefore, an in silico approach was developed by refitting the double two-st