Topic: paracellular permeability

Circadian rhythms are ~24-hour oscillations that provide temporal integration of physiology and behavior that are entrained to the earth’s light-dark cycle via photic input to the suprachiasmatic nuclei (SCN). The SCN (i.e., central clock), sets central and peripheral clocks accordingly to maintain proper synchronization with the external solar day. Exposure to artificial light at night (ALAN) resets the SCN, disrupting daily internal rhythms. Our lab and others have demonstrated daily rhythms in transcellular permeability via efflux mediated transport at the blood-brain barrier (BBB). However

SYNGAP1-related encephalopathy is a rare neurodevelopmental disease characterized by diverse genetic mutations and phenotypic features. It is not known whether the blood-brain barrier (BBB) is affected in SYNGAP1 patients, therefore, this study aimed to establish a human induced pluripotent stem cell (hiPSC)-based BBB model of the SYNGAP1 disorder and identify relevant functional and molecular signatures. To establish the BBB models, SYNGAP1 knockout (KO), patient-derived hiPSC lines (Leu323Arg) and isogenic controls were differentiated into brain capillary endothelial-like cells and seeded on