Topic: patch-sequencing

GABAergic interneurons (INs) and their maturation play a pivotal role in various brain functions, with implications for a wide spectrum of neurological and psychiatric disorders. Among the key regulators of GABAergic inhibition, the cation chloride co-transporter KCC2 is central; influencing interneuronal function, CNS development, plasticity, and disease pathology. While KCC2's role in principal neurons’ transition of GABAergic signaling from depolarizing to hyperpolarizing is well-documented, its expression patterns and functional significance in interneurons, particularly during early neona

Neuropsychiatric disorders, such as schizophrenia, have a strong genetic basis and are linked to structural genomic changes such as copy number variations, including the 15q13.3 microdeletion. This study investigates the impact of the 15q13.3 microdeletion on neuronal maturation in the cerebral cortex, using a mouse model. We performed single-cell transcriptomics to analyse the transcriptomic changes in neuronal subtypes at different maturational stages. Compositional changes were validated with immunohistochemistry, while functional validation was conducted through calcium imaging and Patch-s