Topic: Pax6

The brain undergoes important growth and plasticity during prenatal development. Altered activation of the glucocorticoid receptor (GR) system is one of the factors mediating stress effects during this time, likely through transcriptional dysregulation. To investigate these processes in a human-specific in vitro system, we used induced pluripotent stem cell-derived 3-dimensional brain organoids. To determine cell-type specific GR activation response, we profiled the transcriptomes of thousands of individual cells using single-cell transcriptomic analyses following glucocorticoids exposure acut

Aim: The molecular mechanisms involved in the development and specification of the retinal ganglion cell (RGC) types have been studied thoroughly in animal models, especially mice. In comparison, human RGCs are much less studied. Our aim was to characterize human RGCs anatomy and molecular markers expression at different fetal life stages in comparison to their mouse orthologues. Methods: The study was conducted on human fetal retinas collected from therapeutic abortion specimens under written informed consent and on C57B6/J mouse retinas. Immunofluorescence staining was performed on vertical

The cerebral cortex is one of the brain structures with the highest cell type diversity. Yet the mechanisms underlying this diversity remain elusive. While various parameters such as gene expression and chromatin accessibility can be assessed within these cells, a dimension of variability that has been largely overlooked is isoform diversity. Pax6 is a fundamental transcription factor during development of the nervous system, especially eye and cortical development. It is also a largely studied homeobox gene, but there is limited knowledge about its isoforms and their specific roles. We studie