Topic: perivascular space

Malignant peripheral trigeminal sheath tumor (MPNST) spreads to the brain parenchyma via the perivascular space (PVS). Nestin-expressing neoplastic cells lead tumor progression. Tumor-associated macrophages and microglia (TAMs) are a critical immune cells population for promoting this progression. In this work, we study the distribution of Nestin+ cells and TAMs in the infiltrative perivascular spaces of endogenous rat MPNSTs.MPNSTs are induced in Sprague Dawley rats by prenatal exposure of N-Ethyl N-nitrosourea (ENU). Tumor screening is performed by T1-w Magnetic Resonance Imaging and by immu

Neurons and glial cells are surrounded by interstitial fluid (ISF), which primarily originates from cerebrospinal fluid (CSF). The fluid dynamics of ISF and CSF have been examined by cisterna magna injection of fluid tracers such as fluorescent dextran or contrast agents for magnetic resonance imaging. While this approach has unveiled major CSF flow dynamics in the recent decade, cisterna magna injection introduces transient intracranial pressure changes that can lead to experimental variability. In this study, we attempted to label the CSF/ISF via endogenously expressed secretory fluorescent