Topic: Pons

Astrocyte calcium signals are region-specific and state-dependent across the sleep-wake cycle. However, their role in sleep regulation remains poorly understood. Since the pons is critical for sleep, especially rapid eye movement (REM) sleep regulation, we investigate the dynamics of pontine astrocyte calcium signals and their role in REM sleep induction in mice. We monitored pontine astrocyte calcium signals by expressing GCaMP6f under the control of gfaABC1D promoter and implanted optic fiber into the sublaterodorsal tegmental nucleus (SLD). Also, we simultaneously acquired cortical electroe