Topic: Primary neuronal cultures

The application of biotin ligase or peroxidase fused with specific proteins or subcellular regions, combined with mass spectrometry-based quantitative proteomics, has become a potent approach for unravelling molecular interactions and determining the locations of native proteins based on their proximity. Recent applications of proximity-dependent biotinylation in neuroscience have yielded insights into the composition of sub-synaptic structures, including the synaptic cleft [1].Despite the effectiveness of proximity labelling methods in providing a global perspective on protein-protein interac

Central nervous system (CNS) neurons have a limited capacity to spontaneously regenerate following injury and in neurodegenerative diseases, which calls for new pro-neurogenic strategies to counteract neurodegeneration. Therefore, understanding neuronal differentiation, function and plasticity by identifying key factors/modulators is a crucial demand to expand the knowledge on CNS ability to reorganize and adapt in res lesions response. BRI2 is a transmembrane protein, which undergoes proteolytic processing, resulting in generation of several secreted peptides whose function remains unknown. P

Cell culture is a fundamental experimental tool for understanding cell physiology. However, translating these findings to in vivo settings has proven challenging. Replicating donor tissue conditions, including oxygen levels, is crucial for achieving meaningful results. Nevertheless, oxygen culture conditions are often overlooked, particularly in the context of chemotherapy-induced neurotoxicity. In this study we want to investigate the role of oxygen levels in primary neuronal cultures by comparing neuron performance under cisplatin exposure (1 µg/ml) in normoxia (representing atmospheric cond