Topic: primary rat neurons

How the brain processes information to learn and form memories is still a widely unanswered question. To gain insights, neurons are studied under great effort in vivo. However, the applied methods often only enable monitoring subsets of neurons embedded in a complex network or lack spatiotemporal resolution for analysis on a single-cell level. In bottom-up neuroscience, small neural networks of reduced complexity are engineered by seeding primary rat neurons or human induced pluripotent stem cell derived neurons into polydimethylsiloxane microstructures on top of microelectrode arrays (MEAs) t

Therapies for neurodegenerative disease are limited in efficacy and tolerability, largely due to poor drug penetration across the BBB and off-target effects. Polymeric nanoparticles (NPs) are advantageous for brain drug delivery due to their safety profiles, drug-loading capacity, controlled-release properties and potential for drug repurposing. The aim of this work was to perform in vitro screening of NPs synthesised using the FDA-approved poly(lactic co-glycolic acid) (PLGA) polymer as drug delivery vehicles in primary rat neurons and glia as a prerequisite for further assessment in animal m